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rabbit anti-glur4  (Millipore)


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    Structured Review

    Millipore rabbit anti-glur4
    Rabbit Anti Glur4, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-glur4/anti+glur4/pm37328451-492-28-30
    Average 90 stars, based on 1 article reviews
    rabbit anti-glur4 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Immunolabeling:

    Article Title: Activity-Dependent Gating of Parvalbumin Interneuron Function by the Perineuronal Net Protein Brevican.
    Article Snippet: The lysate was then diluted in 1 mL of coIP buffer (0.3M sucrose, 10 mM Tris-HCl pH 8, 10 mMNaCl, 3 mMMgCl2, 1%NP-40, 5% glycerol and protease inhibitor cocktail) and subsequently incubated overnight at 4 C with 2 mg of one of the following antibodies: rabbit anti-GluR1 (Millipore #04-855), rabbit anti-GluR2-3 (Millipore #AB1506), rabbit anti-GluR4 (Millipore #AB1508), rabbit anti-NMDAR2B (Millipore #AB1557P), Rabbit IgG-Isotype Control (Abcam #ab27478), rabbit anti-mGluR2-3 (Millipore #AB1553), mouse anti-Kv1.1 (NeuroMab #75-007), mouse anti-Kv3.1b (NeuroMab #73-041), Mouse IgG-Isotype Control (Abcam # ab37355).

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore),

    Article Title: Synergistic Effect of Perampanel and Temozolomide in Human Glioma Cell Lines
    Article Snippet: In total, 150,000 cells/250 μL were incubated for 1 h at 4 °C, with 1 μg of the following primary antibodies: rabbit anti-GluR1, rabbit anti-GluR2&3, and rabbit anti-GluR4 (Chemicon, Temecula, CA, USA).

    Article Title: Altered sensitivity to excitotoxic cell death and glutamate receptor expression between two commonly studied mouse strains
    Article Snippet: Membranes were then incubated with antibodies to either AMPA receptor subunits [rabbit anti-GluR1 (Cat. No. AB1504), 1:1000; rabbit anti-GluR2 (Cat. No. AB1768), 1:2000; rabbit anti-GluR4 (Cat. No. 06-308), 1:1000; all from Millipore (Temecula, CA)] or NMDA receptor subunits [goat anti-NR2A (Cat. No. sc-1468), 1:1000; goat anti-NR2B (Cat. No. sc-1469), 1:500; both from Santa Cruz Biotech. (Santa Cruz, CA)] in TBS-T containing 5% nonfat dry milk at 4 °C for 65–68 h. In the case when β–tubulin was also detected, the membrane was horizontally cut immediately after the protein transfer, and the membrane piece containing the proteins with molecular weight below 70 kDa was incubated with a rabbit anti-β–tubulin antibody (1:20000; Sigma, St. Louis, MO).

    Article Title: Diabetes changes the levels of ionotropic glutamate receptors in the rat retina
    Article Snippet: Rabbit anti-GluR4 , 1:500 , Chemicon.

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Antibodies Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore), Microscopy TIRF microscopy was done on an Olympus 1X71 equipped with a 60x TIRF objective.

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse.
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma,HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/ Cascade Blue, A-5760 (1:500).

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma, HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/Cascade Blue, A-5760 (1:500).

    Labeling:

    Article Title: Activity-Dependent Gating of Parvalbumin Interneuron Function by the Perineuronal Net Protein Brevican.
    Article Snippet: The lysate was then diluted in 1 mL of coIP buffer (0.3M sucrose, 10 mM Tris-HCl pH 8, 10 mMNaCl, 3 mMMgCl2, 1%NP-40, 5% glycerol and protease inhibitor cocktail) and subsequently incubated overnight at 4 C with 2 mg of one of the following antibodies: rabbit anti-GluR1 (Millipore #04-855), rabbit anti-GluR2-3 (Millipore #AB1506), rabbit anti-GluR4 (Millipore #AB1508), rabbit anti-NMDAR2B (Millipore #AB1557P), Rabbit IgG-Isotype Control (Abcam #ab27478), rabbit anti-mGluR2-3 (Millipore #AB1553), mouse anti-Kv1.1 (NeuroMab #75-007), mouse anti-Kv3.1b (NeuroMab #73-041), Mouse IgG-Isotype Control (Abcam # ab37355).

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore),

    Article Title: Synergistic Effect of Perampanel and Temozolomide in Human Glioma Cell Lines
    Article Snippet: In total, 150,000 cells/250 μL were incubated for 1 h at 4 °C, with 1 μg of the following primary antibodies: rabbit anti-GluR1, rabbit anti-GluR2&3, and rabbit anti-GluR4 (Chemicon, Temecula, CA, USA).

    Article Title: Altered sensitivity to excitotoxic cell death and glutamate receptor expression between two commonly studied mouse strains
    Article Snippet: Membranes were then incubated with antibodies to either AMPA receptor subunits [rabbit anti-GluR1 (Cat. No. AB1504), 1:1000; rabbit anti-GluR2 (Cat. No. AB1768), 1:2000; rabbit anti-GluR4 (Cat. No. 06-308), 1:1000; all from Millipore (Temecula, CA)] or NMDA receptor subunits [goat anti-NR2A (Cat. No. sc-1468), 1:1000; goat anti-NR2B (Cat. No. sc-1469), 1:500; both from Santa Cruz Biotech. (Santa Cruz, CA)] in TBS-T containing 5% nonfat dry milk at 4 °C for 65–68 h. In the case when β–tubulin was also detected, the membrane was horizontally cut immediately after the protein transfer, and the membrane piece containing the proteins with molecular weight below 70 kDa was incubated with a rabbit anti-β–tubulin antibody (1:20000; Sigma, St. Louis, MO).

    Article Title: Diabetes changes the levels of ionotropic glutamate receptors in the rat retina
    Article Snippet: Rabbit anti-GluR4 , 1:500 , Chemicon.

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Antibodies Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore), Microscopy TIRF microscopy was done on an Olympus 1X71 equipped with a 60x TIRF objective.

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse.
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma,HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/ Cascade Blue, A-5760 (1:500).

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma, HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/Cascade Blue, A-5760 (1:500).

    Expressing:

    Article Title: Activity-Dependent Gating of Parvalbumin Interneuron Function by the Perineuronal Net Protein Brevican.
    Article Snippet: The lysate was then diluted in 1 mL of coIP buffer (0.3M sucrose, 10 mM Tris-HCl pH 8, 10 mMNaCl, 3 mMMgCl2, 1%NP-40, 5% glycerol and protease inhibitor cocktail) and subsequently incubated overnight at 4 C with 2 mg of one of the following antibodies: rabbit anti-GluR1 (Millipore #04-855), rabbit anti-GluR2-3 (Millipore #AB1506), rabbit anti-GluR4 (Millipore #AB1508), rabbit anti-NMDAR2B (Millipore #AB1557P), Rabbit IgG-Isotype Control (Abcam #ab27478), rabbit anti-mGluR2-3 (Millipore #AB1553), mouse anti-Kv1.1 (NeuroMab #75-007), mouse anti-Kv3.1b (NeuroMab #73-041), Mouse IgG-Isotype Control (Abcam # ab37355).

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore),

    Article Title: Synergistic Effect of Perampanel and Temozolomide in Human Glioma Cell Lines
    Article Snippet: In total, 150,000 cells/250 μL were incubated for 1 h at 4 °C, with 1 μg of the following primary antibodies: rabbit anti-GluR1, rabbit anti-GluR2&3, and rabbit anti-GluR4 (Chemicon, Temecula, CA, USA).

    Article Title: Altered sensitivity to excitotoxic cell death and glutamate receptor expression between two commonly studied mouse strains
    Article Snippet: Membranes were then incubated with antibodies to either AMPA receptor subunits [rabbit anti-GluR1 (Cat. No. AB1504), 1:1000; rabbit anti-GluR2 (Cat. No. AB1768), 1:2000; rabbit anti-GluR4 (Cat. No. 06-308), 1:1000; all from Millipore (Temecula, CA)] or NMDA receptor subunits [goat anti-NR2A (Cat. No. sc-1468), 1:1000; goat anti-NR2B (Cat. No. sc-1469), 1:500; both from Santa Cruz Biotech. (Santa Cruz, CA)] in TBS-T containing 5% nonfat dry milk at 4 °C for 65–68 h. In the case when β–tubulin was also detected, the membrane was horizontally cut immediately after the protein transfer, and the membrane piece containing the proteins with molecular weight below 70 kDa was incubated with a rabbit anti-β–tubulin antibody (1:20000; Sigma, St. Louis, MO).

    Article Title: Diabetes changes the levels of ionotropic glutamate receptors in the rat retina
    Article Snippet: Rabbit anti-GluR4 , 1:500 , Chemicon.

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Antibodies Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore), Microscopy TIRF microscopy was done on an Olympus 1X71 equipped with a 60x TIRF objective.

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse.
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma,HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/ Cascade Blue, A-5760 (1:500).

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma, HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/Cascade Blue, A-5760 (1:500).

    Marker:

    Article Title: Activity-Dependent Gating of Parvalbumin Interneuron Function by the Perineuronal Net Protein Brevican.
    Article Snippet: The lysate was then diluted in 1 mL of coIP buffer (0.3M sucrose, 10 mM Tris-HCl pH 8, 10 mMNaCl, 3 mMMgCl2, 1%NP-40, 5% glycerol and protease inhibitor cocktail) and subsequently incubated overnight at 4 C with 2 mg of one of the following antibodies: rabbit anti-GluR1 (Millipore #04-855), rabbit anti-GluR2-3 (Millipore #AB1506), rabbit anti-GluR4 (Millipore #AB1508), rabbit anti-NMDAR2B (Millipore #AB1557P), Rabbit IgG-Isotype Control (Abcam #ab27478), rabbit anti-mGluR2-3 (Millipore #AB1553), mouse anti-Kv1.1 (NeuroMab #75-007), mouse anti-Kv3.1b (NeuroMab #73-041), Mouse IgG-Isotype Control (Abcam # ab37355).

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore),

    Article Title: Synergistic Effect of Perampanel and Temozolomide in Human Glioma Cell Lines
    Article Snippet: In total, 150,000 cells/250 μL were incubated for 1 h at 4 °C, with 1 μg of the following primary antibodies: rabbit anti-GluR1, rabbit anti-GluR2&3, and rabbit anti-GluR4 (Chemicon, Temecula, CA, USA).

    Article Title: Altered sensitivity to excitotoxic cell death and glutamate receptor expression between two commonly studied mouse strains
    Article Snippet: Membranes were then incubated with antibodies to either AMPA receptor subunits [rabbit anti-GluR1 (Cat. No. AB1504), 1:1000; rabbit anti-GluR2 (Cat. No. AB1768), 1:2000; rabbit anti-GluR4 (Cat. No. 06-308), 1:1000; all from Millipore (Temecula, CA)] or NMDA receptor subunits [goat anti-NR2A (Cat. No. sc-1468), 1:1000; goat anti-NR2B (Cat. No. sc-1469), 1:500; both from Santa Cruz Biotech. (Santa Cruz, CA)] in TBS-T containing 5% nonfat dry milk at 4 °C for 65–68 h. In the case when β–tubulin was also detected, the membrane was horizontally cut immediately after the protein transfer, and the membrane piece containing the proteins with molecular weight below 70 kDa was incubated with a rabbit anti-β–tubulin antibody (1:20000; Sigma, St. Louis, MO).

    Article Title: Diabetes changes the levels of ionotropic glutamate receptors in the rat retina
    Article Snippet: Rabbit anti-GluR4 , 1:500 , Chemicon.

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Antibodies Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore), Microscopy TIRF microscopy was done on an Olympus 1X71 equipped with a 60x TIRF objective.

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse.
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma,HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/ Cascade Blue, A-5760 (1:500).

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma, HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/Cascade Blue, A-5760 (1:500).

    Binding Assay:

    Article Title: Activity-Dependent Gating of Parvalbumin Interneuron Function by the Perineuronal Net Protein Brevican.
    Article Snippet: The lysate was then diluted in 1 mL of coIP buffer (0.3M sucrose, 10 mM Tris-HCl pH 8, 10 mMNaCl, 3 mMMgCl2, 1%NP-40, 5% glycerol and protease inhibitor cocktail) and subsequently incubated overnight at 4 C with 2 mg of one of the following antibodies: rabbit anti-GluR1 (Millipore #04-855), rabbit anti-GluR2-3 (Millipore #AB1506), rabbit anti-GluR4 (Millipore #AB1508), rabbit anti-NMDAR2B (Millipore #AB1557P), Rabbit IgG-Isotype Control (Abcam #ab27478), rabbit anti-mGluR2-3 (Millipore #AB1553), mouse anti-Kv1.1 (NeuroMab #75-007), mouse anti-Kv3.1b (NeuroMab #73-041), Mouse IgG-Isotype Control (Abcam # ab37355).

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore),

    Article Title: Synergistic Effect of Perampanel and Temozolomide in Human Glioma Cell Lines
    Article Snippet: In total, 150,000 cells/250 μL were incubated for 1 h at 4 °C, with 1 μg of the following primary antibodies: rabbit anti-GluR1, rabbit anti-GluR2&3, and rabbit anti-GluR4 (Chemicon, Temecula, CA, USA).

    Article Title: Altered sensitivity to excitotoxic cell death and glutamate receptor expression between two commonly studied mouse strains
    Article Snippet: Membranes were then incubated with antibodies to either AMPA receptor subunits [rabbit anti-GluR1 (Cat. No. AB1504), 1:1000; rabbit anti-GluR2 (Cat. No. AB1768), 1:2000; rabbit anti-GluR4 (Cat. No. 06-308), 1:1000; all from Millipore (Temecula, CA)] or NMDA receptor subunits [goat anti-NR2A (Cat. No. sc-1468), 1:1000; goat anti-NR2B (Cat. No. sc-1469), 1:500; both from Santa Cruz Biotech. (Santa Cruz, CA)] in TBS-T containing 5% nonfat dry milk at 4 °C for 65–68 h. In the case when β–tubulin was also detected, the membrane was horizontally cut immediately after the protein transfer, and the membrane piece containing the proteins with molecular weight below 70 kDa was incubated with a rabbit anti-β–tubulin antibody (1:20000; Sigma, St. Louis, MO).

    Article Title: Diabetes changes the levels of ionotropic glutamate receptors in the rat retina
    Article Snippet: Rabbit anti-GluR4 , 1:500 , Chemicon.

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Antibodies Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore), Microscopy TIRF microscopy was done on an Olympus 1X71 equipped with a 60x TIRF objective.

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse.
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma,HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/ Cascade Blue, A-5760 (1:500).

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma, HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/Cascade Blue, A-5760 (1:500).

    Western Blot:

    Article Title: Activity-Dependent Gating of Parvalbumin Interneuron Function by the Perineuronal Net Protein Brevican.
    Article Snippet: The lysate was then diluted in 1 mL of coIP buffer (0.3M sucrose, 10 mM Tris-HCl pH 8, 10 mMNaCl, 3 mMMgCl2, 1%NP-40, 5% glycerol and protease inhibitor cocktail) and subsequently incubated overnight at 4 C with 2 mg of one of the following antibodies: rabbit anti-GluR1 (Millipore #04-855), rabbit anti-GluR2-3 (Millipore #AB1506), rabbit anti-GluR4 (Millipore #AB1508), rabbit anti-NMDAR2B (Millipore #AB1557P), Rabbit IgG-Isotype Control (Abcam #ab27478), rabbit anti-mGluR2-3 (Millipore #AB1553), mouse anti-Kv1.1 (NeuroMab #75-007), mouse anti-Kv3.1b (NeuroMab #73-041), Mouse IgG-Isotype Control (Abcam # ab37355).

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore),

    Article Title: Synergistic Effect of Perampanel and Temozolomide in Human Glioma Cell Lines
    Article Snippet: In total, 150,000 cells/250 μL were incubated for 1 h at 4 °C, with 1 μg of the following primary antibodies: rabbit anti-GluR1, rabbit anti-GluR2&3, and rabbit anti-GluR4 (Chemicon, Temecula, CA, USA).

    Article Title: Altered sensitivity to excitotoxic cell death and glutamate receptor expression between two commonly studied mouse strains
    Article Snippet: Membranes were then incubated with antibodies to either AMPA receptor subunits [rabbit anti-GluR1 (Cat. No. AB1504), 1:1000; rabbit anti-GluR2 (Cat. No. AB1768), 1:2000; rabbit anti-GluR4 (Cat. No. 06-308), 1:1000; all from Millipore (Temecula, CA)] or NMDA receptor subunits [goat anti-NR2A (Cat. No. sc-1468), 1:1000; goat anti-NR2B (Cat. No. sc-1469), 1:500; both from Santa Cruz Biotech. (Santa Cruz, CA)] in TBS-T containing 5% nonfat dry milk at 4 °C for 65–68 h. In the case when β–tubulin was also detected, the membrane was horizontally cut immediately after the protein transfer, and the membrane piece containing the proteins with molecular weight below 70 kDa was incubated with a rabbit anti-β–tubulin antibody (1:20000; Sigma, St. Louis, MO).

    Article Title: Diabetes changes the levels of ionotropic glutamate receptors in the rat retina
    Article Snippet: Rabbit anti-GluR4 , 1:500 , Chemicon.

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Antibodies Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore), Microscopy TIRF microscopy was done on an Olympus 1X71 equipped with a 60x TIRF objective.

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse.
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma,HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/ Cascade Blue, A-5760 (1:500).

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma, HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/Cascade Blue, A-5760 (1:500).

    Two Tailed Test:

    Article Title: Activity-Dependent Gating of Parvalbumin Interneuron Function by the Perineuronal Net Protein Brevican.
    Article Snippet: The lysate was then diluted in 1 mL of coIP buffer (0.3M sucrose, 10 mM Tris-HCl pH 8, 10 mMNaCl, 3 mMMgCl2, 1%NP-40, 5% glycerol and protease inhibitor cocktail) and subsequently incubated overnight at 4 C with 2 mg of one of the following antibodies: rabbit anti-GluR1 (Millipore #04-855), rabbit anti-GluR2-3 (Millipore #AB1506), rabbit anti-GluR4 (Millipore #AB1508), rabbit anti-NMDAR2B (Millipore #AB1557P), Rabbit IgG-Isotype Control (Abcam #ab27478), rabbit anti-mGluR2-3 (Millipore #AB1553), mouse anti-Kv1.1 (NeuroMab #75-007), mouse anti-Kv3.1b (NeuroMab #73-041), Mouse IgG-Isotype Control (Abcam # ab37355).

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore),

    Article Title: Synergistic Effect of Perampanel and Temozolomide in Human Glioma Cell Lines
    Article Snippet: In total, 150,000 cells/250 μL were incubated for 1 h at 4 °C, with 1 μg of the following primary antibodies: rabbit anti-GluR1, rabbit anti-GluR2&3, and rabbit anti-GluR4 (Chemicon, Temecula, CA, USA).

    Article Title: Altered sensitivity to excitotoxic cell death and glutamate receptor expression between two commonly studied mouse strains
    Article Snippet: Membranes were then incubated with antibodies to either AMPA receptor subunits [rabbit anti-GluR1 (Cat. No. AB1504), 1:1000; rabbit anti-GluR2 (Cat. No. AB1768), 1:2000; rabbit anti-GluR4 (Cat. No. 06-308), 1:1000; all from Millipore (Temecula, CA)] or NMDA receptor subunits [goat anti-NR2A (Cat. No. sc-1468), 1:1000; goat anti-NR2B (Cat. No. sc-1469), 1:500; both from Santa Cruz Biotech. (Santa Cruz, CA)] in TBS-T containing 5% nonfat dry milk at 4 °C for 65–68 h. In the case when β–tubulin was also detected, the membrane was horizontally cut immediately after the protein transfer, and the membrane piece containing the proteins with molecular weight below 70 kDa was incubated with a rabbit anti-β–tubulin antibody (1:20000; Sigma, St. Louis, MO).

    Article Title: Diabetes changes the levels of ionotropic glutamate receptors in the rat retina
    Article Snippet: Rabbit anti-GluR4 , 1:500 , Chemicon.

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Antibodies Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore), Microscopy TIRF microscopy was done on an Olympus 1X71 equipped with a 60x TIRF objective.

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse.
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma,HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/ Cascade Blue, A-5760 (1:500).

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma, HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/Cascade Blue, A-5760 (1:500).

    Isolation:

    Article Title: Activity-Dependent Gating of Parvalbumin Interneuron Function by the Perineuronal Net Protein Brevican.
    Article Snippet: The lysate was then diluted in 1 mL of coIP buffer (0.3M sucrose, 10 mM Tris-HCl pH 8, 10 mMNaCl, 3 mMMgCl2, 1%NP-40, 5% glycerol and protease inhibitor cocktail) and subsequently incubated overnight at 4 C with 2 mg of one of the following antibodies: rabbit anti-GluR1 (Millipore #04-855), rabbit anti-GluR2-3 (Millipore #AB1506), rabbit anti-GluR4 (Millipore #AB1508), rabbit anti-NMDAR2B (Millipore #AB1557P), Rabbit IgG-Isotype Control (Abcam #ab27478), rabbit anti-mGluR2-3 (Millipore #AB1553), mouse anti-Kv1.1 (NeuroMab #75-007), mouse anti-Kv3.1b (NeuroMab #73-041), Mouse IgG-Isotype Control (Abcam # ab37355).

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore),

    Article Title: Synergistic Effect of Perampanel and Temozolomide in Human Glioma Cell Lines
    Article Snippet: In total, 150,000 cells/250 μL were incubated for 1 h at 4 °C, with 1 μg of the following primary antibodies: rabbit anti-GluR1, rabbit anti-GluR2&3, and rabbit anti-GluR4 (Chemicon, Temecula, CA, USA).

    Article Title: Altered sensitivity to excitotoxic cell death and glutamate receptor expression between two commonly studied mouse strains
    Article Snippet: Membranes were then incubated with antibodies to either AMPA receptor subunits [rabbit anti-GluR1 (Cat. No. AB1504), 1:1000; rabbit anti-GluR2 (Cat. No. AB1768), 1:2000; rabbit anti-GluR4 (Cat. No. 06-308), 1:1000; all from Millipore (Temecula, CA)] or NMDA receptor subunits [goat anti-NR2A (Cat. No. sc-1468), 1:1000; goat anti-NR2B (Cat. No. sc-1469), 1:500; both from Santa Cruz Biotech. (Santa Cruz, CA)] in TBS-T containing 5% nonfat dry milk at 4 °C for 65–68 h. In the case when β–tubulin was also detected, the membrane was horizontally cut immediately after the protein transfer, and the membrane piece containing the proteins with molecular weight below 70 kDa was incubated with a rabbit anti-β–tubulin antibody (1:20000; Sigma, St. Louis, MO).

    Article Title: Diabetes changes the levels of ionotropic glutamate receptors in the rat retina
    Article Snippet: Rabbit anti-GluR4 , 1:500 , Chemicon.

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Antibodies Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore), Microscopy TIRF microscopy was done on an Olympus 1X71 equipped with a 60x TIRF objective.

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse.
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma,HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/ Cascade Blue, A-5760 (1:500).

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma, HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/Cascade Blue, A-5760 (1:500).

    Protease Inhibitor:

    Article Title: Activity-Dependent Gating of Parvalbumin Interneuron Function by the Perineuronal Net Protein Brevican.
    Article Snippet: The lysate was then diluted in 1 mL of coIP buffer (0.3M sucrose, 10 mM Tris-HCl pH 8, 10 mMNaCl, 3 mMMgCl2, 1%NP-40, 5% glycerol and protease inhibitor cocktail) and subsequently incubated overnight at 4 C with 2 mg of one of the following antibodies: rabbit anti-GluR1 (Millipore #04-855), rabbit anti-GluR2-3 (Millipore #AB1506), rabbit anti-GluR4 (Millipore #AB1508), rabbit anti-NMDAR2B (Millipore #AB1557P), Rabbit IgG-Isotype Control (Abcam #ab27478), rabbit anti-mGluR2-3 (Millipore #AB1553), mouse anti-Kv1.1 (NeuroMab #75-007), mouse anti-Kv3.1b (NeuroMab #73-041), Mouse IgG-Isotype Control (Abcam # ab37355).

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore),

    Article Title: Synergistic Effect of Perampanel and Temozolomide in Human Glioma Cell Lines
    Article Snippet: In total, 150,000 cells/250 μL were incubated for 1 h at 4 °C, with 1 μg of the following primary antibodies: rabbit anti-GluR1, rabbit anti-GluR2&3, and rabbit anti-GluR4 (Chemicon, Temecula, CA, USA).

    Article Title: Altered sensitivity to excitotoxic cell death and glutamate receptor expression between two commonly studied mouse strains
    Article Snippet: Membranes were then incubated with antibodies to either AMPA receptor subunits [rabbit anti-GluR1 (Cat. No. AB1504), 1:1000; rabbit anti-GluR2 (Cat. No. AB1768), 1:2000; rabbit anti-GluR4 (Cat. No. 06-308), 1:1000; all from Millipore (Temecula, CA)] or NMDA receptor subunits [goat anti-NR2A (Cat. No. sc-1468), 1:1000; goat anti-NR2B (Cat. No. sc-1469), 1:500; both from Santa Cruz Biotech. (Santa Cruz, CA)] in TBS-T containing 5% nonfat dry milk at 4 °C for 65–68 h. In the case when β–tubulin was also detected, the membrane was horizontally cut immediately after the protein transfer, and the membrane piece containing the proteins with molecular weight below 70 kDa was incubated with a rabbit anti-β–tubulin antibody (1:20000; Sigma, St. Louis, MO).

    Article Title: Diabetes changes the levels of ionotropic glutamate receptors in the rat retina
    Article Snippet: Rabbit anti-GluR4 , 1:500 , Chemicon.

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Antibodies Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore), Microscopy TIRF microscopy was done on an Olympus 1X71 equipped with a 60x TIRF objective.

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse.
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma,HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/ Cascade Blue, A-5760 (1:500).

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma, HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/Cascade Blue, A-5760 (1:500).

    Incubation:

    Article Title: Activity-Dependent Gating of Parvalbumin Interneuron Function by the Perineuronal Net Protein Brevican.
    Article Snippet: The lysate was then diluted in 1 mL of coIP buffer (0.3M sucrose, 10 mM Tris-HCl pH 8, 10 mMNaCl, 3 mMMgCl2, 1%NP-40, 5% glycerol and protease inhibitor cocktail) and subsequently incubated overnight at 4 C with 2 mg of one of the following antibodies: rabbit anti-GluR1 (Millipore #04-855), rabbit anti-GluR2-3 (Millipore #AB1506), rabbit anti-GluR4 (Millipore #AB1508), rabbit anti-NMDAR2B (Millipore #AB1557P), Rabbit IgG-Isotype Control (Abcam #ab27478), rabbit anti-mGluR2-3 (Millipore #AB1553), mouse anti-Kv1.1 (NeuroMab #75-007), mouse anti-Kv3.1b (NeuroMab #73-041), Mouse IgG-Isotype Control (Abcam # ab37355).

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore),

    Article Title: Synergistic Effect of Perampanel and Temozolomide in Human Glioma Cell Lines
    Article Snippet: In total, 150,000 cells/250 μL were incubated for 1 h at 4 °C, with 1 μg of the following primary antibodies: rabbit anti-GluR1, rabbit anti-GluR2&3, and rabbit anti-GluR4 (Chemicon, Temecula, CA, USA).

    Article Title: Altered sensitivity to excitotoxic cell death and glutamate receptor expression between two commonly studied mouse strains
    Article Snippet: Membranes were then incubated with antibodies to either AMPA receptor subunits [rabbit anti-GluR1 (Cat. No. AB1504), 1:1000; rabbit anti-GluR2 (Cat. No. AB1768), 1:2000; rabbit anti-GluR4 (Cat. No. 06-308), 1:1000; all from Millipore (Temecula, CA)] or NMDA receptor subunits [goat anti-NR2A (Cat. No. sc-1468), 1:1000; goat anti-NR2B (Cat. No. sc-1469), 1:500; both from Santa Cruz Biotech. (Santa Cruz, CA)] in TBS-T containing 5% nonfat dry milk at 4 °C for 65–68 h. In the case when β–tubulin was also detected, the membrane was horizontally cut immediately after the protein transfer, and the membrane piece containing the proteins with molecular weight below 70 kDa was incubated with a rabbit anti-β–tubulin antibody (1:20000; Sigma, St. Louis, MO).

    Article Title: Diabetes changes the levels of ionotropic glutamate receptors in the rat retina
    Article Snippet: Rabbit anti-GluR4 , 1:500 , Chemicon.

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Antibodies Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore), Microscopy TIRF microscopy was done on an Olympus 1X71 equipped with a 60x TIRF objective.

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse.
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma,HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/ Cascade Blue, A-5760 (1:500).

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma, HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/Cascade Blue, A-5760 (1:500).

    Microscopy:

    Article Title: Activity-Dependent Gating of Parvalbumin Interneuron Function by the Perineuronal Net Protein Brevican.
    Article Snippet: The lysate was then diluted in 1 mL of coIP buffer (0.3M sucrose, 10 mM Tris-HCl pH 8, 10 mMNaCl, 3 mMMgCl2, 1%NP-40, 5% glycerol and protease inhibitor cocktail) and subsequently incubated overnight at 4 C with 2 mg of one of the following antibodies: rabbit anti-GluR1 (Millipore #04-855), rabbit anti-GluR2-3 (Millipore #AB1506), rabbit anti-GluR4 (Millipore #AB1508), rabbit anti-NMDAR2B (Millipore #AB1557P), Rabbit IgG-Isotype Control (Abcam #ab27478), rabbit anti-mGluR2-3 (Millipore #AB1553), mouse anti-Kv1.1 (NeuroMab #75-007), mouse anti-Kv3.1b (NeuroMab #73-041), Mouse IgG-Isotype Control (Abcam # ab37355).

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore),

    Article Title: Synergistic Effect of Perampanel and Temozolomide in Human Glioma Cell Lines
    Article Snippet: In total, 150,000 cells/250 μL were incubated for 1 h at 4 °C, with 1 μg of the following primary antibodies: rabbit anti-GluR1, rabbit anti-GluR2&3, and rabbit anti-GluR4 (Chemicon, Temecula, CA, USA).

    Article Title: Altered sensitivity to excitotoxic cell death and glutamate receptor expression between two commonly studied mouse strains
    Article Snippet: Membranes were then incubated with antibodies to either AMPA receptor subunits [rabbit anti-GluR1 (Cat. No. AB1504), 1:1000; rabbit anti-GluR2 (Cat. No. AB1768), 1:2000; rabbit anti-GluR4 (Cat. No. 06-308), 1:1000; all from Millipore (Temecula, CA)] or NMDA receptor subunits [goat anti-NR2A (Cat. No. sc-1468), 1:1000; goat anti-NR2B (Cat. No. sc-1469), 1:500; both from Santa Cruz Biotech. (Santa Cruz, CA)] in TBS-T containing 5% nonfat dry milk at 4 °C for 65–68 h. In the case when β–tubulin was also detected, the membrane was horizontally cut immediately after the protein transfer, and the membrane piece containing the proteins with molecular weight below 70 kDa was incubated with a rabbit anti-β–tubulin antibody (1:20000; Sigma, St. Louis, MO).

    Article Title: Diabetes changes the levels of ionotropic glutamate receptors in the rat retina
    Article Snippet: Rabbit anti-GluR4 , 1:500 , Chemicon.

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Antibodies Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore), Microscopy TIRF microscopy was done on an Olympus 1X71 equipped with a 60x TIRF objective.

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse.
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma,HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/ Cascade Blue, A-5760 (1:500).

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma, HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/Cascade Blue, A-5760 (1:500).

    SDS Page:

    Article Title: Activity-Dependent Gating of Parvalbumin Interneuron Function by the Perineuronal Net Protein Brevican.
    Article Snippet: The lysate was then diluted in 1 mL of coIP buffer (0.3M sucrose, 10 mM Tris-HCl pH 8, 10 mMNaCl, 3 mMMgCl2, 1%NP-40, 5% glycerol and protease inhibitor cocktail) and subsequently incubated overnight at 4 C with 2 mg of one of the following antibodies: rabbit anti-GluR1 (Millipore #04-855), rabbit anti-GluR2-3 (Millipore #AB1506), rabbit anti-GluR4 (Millipore #AB1508), rabbit anti-NMDAR2B (Millipore #AB1557P), Rabbit IgG-Isotype Control (Abcam #ab27478), rabbit anti-mGluR2-3 (Millipore #AB1553), mouse anti-Kv1.1 (NeuroMab #75-007), mouse anti-Kv3.1b (NeuroMab #73-041), Mouse IgG-Isotype Control (Abcam # ab37355).

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore),

    Article Title: Synergistic Effect of Perampanel and Temozolomide in Human Glioma Cell Lines
    Article Snippet: In total, 150,000 cells/250 μL were incubated for 1 h at 4 °C, with 1 μg of the following primary antibodies: rabbit anti-GluR1, rabbit anti-GluR2&3, and rabbit anti-GluR4 (Chemicon, Temecula, CA, USA).

    Article Title: Altered sensitivity to excitotoxic cell death and glutamate receptor expression between two commonly studied mouse strains
    Article Snippet: Membranes were then incubated with antibodies to either AMPA receptor subunits [rabbit anti-GluR1 (Cat. No. AB1504), 1:1000; rabbit anti-GluR2 (Cat. No. AB1768), 1:2000; rabbit anti-GluR4 (Cat. No. 06-308), 1:1000; all from Millipore (Temecula, CA)] or NMDA receptor subunits [goat anti-NR2A (Cat. No. sc-1468), 1:1000; goat anti-NR2B (Cat. No. sc-1469), 1:500; both from Santa Cruz Biotech. (Santa Cruz, CA)] in TBS-T containing 5% nonfat dry milk at 4 °C for 65–68 h. In the case when β–tubulin was also detected, the membrane was horizontally cut immediately after the protein transfer, and the membrane piece containing the proteins with molecular weight below 70 kDa was incubated with a rabbit anti-β–tubulin antibody (1:20000; Sigma, St. Louis, MO).

    Article Title: Diabetes changes the levels of ionotropic glutamate receptors in the rat retina
    Article Snippet: Rabbit anti-GluR4 , 1:500 , Chemicon.

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Antibodies Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore), Microscopy TIRF microscopy was done on an Olympus 1X71 equipped with a 60x TIRF objective.

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse.
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma,HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/ Cascade Blue, A-5760 (1:500).

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma, HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/Cascade Blue, A-5760 (1:500).

    Membrane:

    Article Title: Activity-Dependent Gating of Parvalbumin Interneuron Function by the Perineuronal Net Protein Brevican.
    Article Snippet: The lysate was then diluted in 1 mL of coIP buffer (0.3M sucrose, 10 mM Tris-HCl pH 8, 10 mMNaCl, 3 mMMgCl2, 1%NP-40, 5% glycerol and protease inhibitor cocktail) and subsequently incubated overnight at 4 C with 2 mg of one of the following antibodies: rabbit anti-GluR1 (Millipore #04-855), rabbit anti-GluR2-3 (Millipore #AB1506), rabbit anti-GluR4 (Millipore #AB1508), rabbit anti-NMDAR2B (Millipore #AB1557P), Rabbit IgG-Isotype Control (Abcam #ab27478), rabbit anti-mGluR2-3 (Millipore #AB1553), mouse anti-Kv1.1 (NeuroMab #75-007), mouse anti-Kv3.1b (NeuroMab #73-041), Mouse IgG-Isotype Control (Abcam # ab37355).

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore),

    Article Title: Synergistic Effect of Perampanel and Temozolomide in Human Glioma Cell Lines
    Article Snippet: In total, 150,000 cells/250 μL were incubated for 1 h at 4 °C, with 1 μg of the following primary antibodies: rabbit anti-GluR1, rabbit anti-GluR2&3, and rabbit anti-GluR4 (Chemicon, Temecula, CA, USA).

    Article Title: Altered sensitivity to excitotoxic cell death and glutamate receptor expression between two commonly studied mouse strains
    Article Snippet: Membranes were then incubated with antibodies to either AMPA receptor subunits [rabbit anti-GluR1 (Cat. No. AB1504), 1:1000; rabbit anti-GluR2 (Cat. No. AB1768), 1:2000; rabbit anti-GluR4 (Cat. No. 06-308), 1:1000; all from Millipore (Temecula, CA)] or NMDA receptor subunits [goat anti-NR2A (Cat. No. sc-1468), 1:1000; goat anti-NR2B (Cat. No. sc-1469), 1:500; both from Santa Cruz Biotech. (Santa Cruz, CA)] in TBS-T containing 5% nonfat dry milk at 4 °C for 65–68 h. In the case when β–tubulin was also detected, the membrane was horizontally cut immediately after the protein transfer, and the membrane piece containing the proteins with molecular weight below 70 kDa was incubated with a rabbit anti-β–tubulin antibody (1:20000; Sigma, St. Louis, MO).

    Article Title: Diabetes changes the levels of ionotropic glutamate receptors in the rat retina
    Article Snippet: Rabbit anti-GluR4 , 1:500 , Chemicon.

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Antibodies Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore), Microscopy TIRF microscopy was done on an Olympus 1X71 equipped with a 60x TIRF objective.

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse.
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma,HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/ Cascade Blue, A-5760 (1:500).

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma, HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/Cascade Blue, A-5760 (1:500).

    Molecular Weight:

    Article Title: Activity-Dependent Gating of Parvalbumin Interneuron Function by the Perineuronal Net Protein Brevican.
    Article Snippet: The lysate was then diluted in 1 mL of coIP buffer (0.3M sucrose, 10 mM Tris-HCl pH 8, 10 mMNaCl, 3 mMMgCl2, 1%NP-40, 5% glycerol and protease inhibitor cocktail) and subsequently incubated overnight at 4 C with 2 mg of one of the following antibodies: rabbit anti-GluR1 (Millipore #04-855), rabbit anti-GluR2-3 (Millipore #AB1506), rabbit anti-GluR4 (Millipore #AB1508), rabbit anti-NMDAR2B (Millipore #AB1557P), Rabbit IgG-Isotype Control (Abcam #ab27478), rabbit anti-mGluR2-3 (Millipore #AB1553), mouse anti-Kv1.1 (NeuroMab #75-007), mouse anti-Kv3.1b (NeuroMab #73-041), Mouse IgG-Isotype Control (Abcam # ab37355).

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore),

    Article Title: Synergistic Effect of Perampanel and Temozolomide in Human Glioma Cell Lines
    Article Snippet: In total, 150,000 cells/250 μL were incubated for 1 h at 4 °C, with 1 μg of the following primary antibodies: rabbit anti-GluR1, rabbit anti-GluR2&3, and rabbit anti-GluR4 (Chemicon, Temecula, CA, USA).

    Article Title: Altered sensitivity to excitotoxic cell death and glutamate receptor expression between two commonly studied mouse strains
    Article Snippet: Membranes were then incubated with antibodies to either AMPA receptor subunits [rabbit anti-GluR1 (Cat. No. AB1504), 1:1000; rabbit anti-GluR2 (Cat. No. AB1768), 1:2000; rabbit anti-GluR4 (Cat. No. 06-308), 1:1000; all from Millipore (Temecula, CA)] or NMDA receptor subunits [goat anti-NR2A (Cat. No. sc-1468), 1:1000; goat anti-NR2B (Cat. No. sc-1469), 1:500; both from Santa Cruz Biotech. (Santa Cruz, CA)] in TBS-T containing 5% nonfat dry milk at 4 °C for 65–68 h. In the case when β–tubulin was also detected, the membrane was horizontally cut immediately after the protein transfer, and the membrane piece containing the proteins with molecular weight below 70 kDa was incubated with a rabbit anti-β–tubulin antibody (1:20000; Sigma, St. Louis, MO).

    Article Title: Diabetes changes the levels of ionotropic glutamate receptors in the rat retina
    Article Snippet: Rabbit anti-GluR4 , 1:500 , Chemicon.

    Article Title: Amphetamine Modulates Glutamatergic Neurotransmission through Endocytosis of the Excitatory Amino Acid Transporter EAAT3 in Dopamine Neurons
    Article Snippet: Antibodies Rabbit anti-EAAT3 (Alpha Diagnostics), mouse anti-TH (Sigma), rat anti-DAT (Chemicon), rabbit anti-EAAT3 (Alpha diagnostics), rabbit anti-EAAT2 (made inhouse), rabbit anti-GluR4 (Millipore) rabbit anti-GluR1 (Upstate), and mouse anti-NR1 (Millipore), Microscopy TIRF microscopy was done on an Olympus 1X71 equipped with a 60x TIRF objective.

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse.
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma,HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/ Cascade Blue, A-5760 (1:500).

    Article Title: Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse
    Article Snippet: Primary antibodies, species, sources, and dilutions include: guinea pig anti-PSD95 PDZ domain, SySy 124014, (dil. 1:1000); rabbit anti-SLC1A7/EAAT5, Sigma, HPA049124, (1:100); goat anti-CtBP2 (C-16), Santa Cruz Biotechnology, sc-5967 (1:100); rabbit anti-GluR4, EMD Millipore, AB1508 (1:200); mouse anti-GluR5 (E-12), Santa Cruz Biotechnology, sc-393420 (1:500); goat anti-ChAT, Chemicon, #AB144P (1:100); chicken anti-GFP, Abcam, ab13970, (1:1000); chicken anti-bassoon, SySy 141016 (1:500); and rabbit anti-Alexa Fluor 405/Cascade Blue, A-5760 (1:500).



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    Image Search Results


    Immunofluorescent visualization of CD11b/c and ionotropic glutamate receptor subunit GluR4 in neonatal rat brain microglia. The integrin CD11b/c and the ionotropic glutamate receptor subunit GluR4 were visualized using the dual-labeling indirect immunofluorescent technique (See Materials and Methods). (Top left) bright field, phase contrast view of microglia incubated in the absence of the primary antibodies against GluR4 and CD11b/c. (Top right) same microglia viewed using FITC filter configuration. Note vesicular structures exhibiting intense autofluorescence, but little or no fluorescence over most of the microglia cell bodies or the elongated processes. (Middle left) bright field, phase contrast view of microglia labeled with anti-glutamate receptor GluR4 subunit and the leukocyte marker CD11b/c. (Middle right)(green) same field viewed using the FITC filter configuration. Microglia not only contain fluorescent vesicles, but exhibit a prominent speckled pattern of fluorescence over the cell bodies and the elongated processes. Absence of speckled pattern of labeling in cells incubated without primary GluR4 antibody (compare with top right photo) suggests the speckled pattern represents specific labeling of GluR4 subunit. (Lower left) same microglia exhibited intense labeling of CD11b/c (red), which confirms microglia are of leukocytic origin. (Bottom right ) GluR4 and CD11b/c superimposed labeling (yellow). Approximate magnification of the original was × 525.

    Journal: BMC Pharmacology

    Article Title: Effect of a short-term in vitro exposure to the marine toxin domoic acid on viability, tumor necrosis factor-alpha, matrix metalloproteinase-9 and superoxide anion release by rat neonatal microglia

    doi: 10.1186/1471-2210-1-7

    Figure Lengend Snippet: Immunofluorescent visualization of CD11b/c and ionotropic glutamate receptor subunit GluR4 in neonatal rat brain microglia. The integrin CD11b/c and the ionotropic glutamate receptor subunit GluR4 were visualized using the dual-labeling indirect immunofluorescent technique (See Materials and Methods). (Top left) bright field, phase contrast view of microglia incubated in the absence of the primary antibodies against GluR4 and CD11b/c. (Top right) same microglia viewed using FITC filter configuration. Note vesicular structures exhibiting intense autofluorescence, but little or no fluorescence over most of the microglia cell bodies or the elongated processes. (Middle left) bright field, phase contrast view of microglia labeled with anti-glutamate receptor GluR4 subunit and the leukocyte marker CD11b/c. (Middle right)(green) same field viewed using the FITC filter configuration. Microglia not only contain fluorescent vesicles, but exhibit a prominent speckled pattern of fluorescence over the cell bodies and the elongated processes. Absence of speckled pattern of labeling in cells incubated without primary GluR4 antibody (compare with top right photo) suggests the speckled pattern represents specific labeling of GluR4 subunit. (Lower left) same microglia exhibited intense labeling of CD11b/c (red), which confirms microglia are of leukocytic origin. (Bottom right ) GluR4 and CD11b/c superimposed labeling (yellow). Approximate magnification of the original was × 525.

    Article Snippet: Hank's balanced salt solution (HBSS), penicillin (P), streptomycin (S), trypsin (0.25%)-EDTA (1 mM) and trypan blue were purchased from GIBCO-BRL (Grand Island, NY); certified heat-inactivated fetal bovine serum (FBS) was obtained from Hyclone (Logan, UT); mouse anti-rat CD11b/c monoclonal antibody (OX-42) and rabbit anti-glutamate receptor (GluR4) polyclonal antibody were purchased from Pharmingen (San Diego, CA).

    Techniques: Labeling, Incubation, Fluorescence, Marker